Odyssey fc manuals, technical notes, white papers, and. These manuals require a valid vin and mailing address. Human anti human actin beta antibody, clone abd12141 recognizes the 42 kda product of the actb gene, cytoplasmic actin beta, also known as actin cytoplasmic 1. I have been working with licor odyssey for last 45 years nowand what i observe is that licor odyssey blocking buffer or licor casein blocking buffer works best and you see less background.
Western system in conjunction with the licor odyssey. The li cor odyssey 9120 imaging system gives your lab clear sharp digital results without the background fluorescence. By using digital images, strong and weak bands are detected on the same. Image studio lite is a free software package from licor biosciences aimed at life scientists that want to analyze gels, western blots, dot. The pearl imager joins licors line up of imaging systems for dna, small animal, and proteomic research including the odyssey imaging system and mousepod, which also offers. Fluorescence wstacker odyssey 9260 for sale biodirect usa. View and download li cor odyssey operators manual online. Used for visualizing images of genes, gene fragments, and gene products. Odyssey clx infrared imaging system operators manual. The licor odyssey 9120 imaging system gives your lab clear sharp digital results without the background fluorescence. The li cor odyssey system is a sensitive far rednear infrared twocolor fluorescence scanner for the imaging and quantitation of gels, blots, 96well plates and a variety of other applications, including tissues and whole animals.
Licor biosciences recommends that all biochemicals be handled carefully, and that safe laboratory procedures be followed at all times. Odyssey clx infrared imaging system from licor biosciences. Licor eddy covariance analyzers have the lowest cost configuration and power requirements among similar instruments. Two different wavelengths for western blot fluorescent dyes 680 and 800nm and pcr gels dyed with gel red detection 600 nm are available. Manuals, software information, and webinars are also listed. Protein detection system is intended for research use only. Which is the best and more sensitive western blot imaging.
A guide to modern quantitative fluorescent western. We have 1 odyssey licor manual available for free pdf download. The licor odyssey system is a sensitive far rednear infrared twocolor fluorescence. Ge typhoon 9410 microarray imager variable mode imager. Electrophoresis membrane transfer blocking antibody addition detection sample prep snap i. Computer software cables cords manual chemiluminescence and. The li cor odyssey fc imaging system is easy to use and the operation is selfexplanatory. The licor odyssey 9120 infrared imaging system passed all tests and was found to be fully functional. Licor biosciences, clx operators manual, clx instructions manual, clx, clx instrument manual created.
The odyssey infrared imaging system allows multiplex western blotting and accurate protein detection and quantification using secondary antibodies labeled with irdye nearinfrared nir fluorescent dyes. Licor biosciences, odyssey infrared imaging system b446. Summary of contents for licor odyssey page 1 the safety of this product can not be guaranteed if the product is used in any other way than is specified in this manual. In addition, software functions related to the odyssey mousepod accessory are discussed in the. Does anybody have experience with blocking reagents. Shown with optional shaker the belly dancer, stovall life science inc. Correct the raw incell elisa signal for the background signal by subtracting the mean signal of wells incubated in the absence of the primary antibody from all other readings. A good place to start is to read the overview of odyssey in this chapter. Licor biosciences, clx operators manual, clx instructions manual, clx, clx instrument manual.
The sapphire biomolecular imager quantitative western. We demod the licor odyssey, biorad chemidoc, and the syngene pxi. The page provides a catalogue of brands and devices, each offering to view or download an updated manual. Prepare samples and determine protein concentrations 2. Li cor biosciences, clx operators manual, clx instructions manual, clx, clx instrument manual created date.
Licor, odyssey infrared imaging system detecting infrared excited and emitted signals high sn ratio from membranes of western blots, protein arrays, micorplates of incell western assays, oncell western assays, gels of dna, protein, coomassiestained, tissue section imaging,whole organ imaging, small animal imaging, elisas. Licor odyssey is an infrared imaging system that is able to eliminate background fluorescence from samples which. The safety of this product can not be guaranteed if the product is used in any other way than is specified in this manual. Anti human actin beta antibody, clone abd12141 biorad. The li cor odyssey 9120 infrared imaging system passed all tests and was found to be fully functional. Page 6 streptavidin incubation wash scan blot on odyssey chapter 3 of the odyssey operators manual describes how to place the blot on the odyssey scanning surface. Our school recently purchased a western blot documentation system to start slowly moving away from film. The odyssey infrared imaging system weighs approximately 33 kg 72 lb. The availability of licor odyssey antibodies makes. Li cor odyssey is an infrared imaging system that is able to eliminate background fluorescence from samples which. Whatever your imaging application the sapphire biomolecular imager and the powerful sapphire capture software will deliver high quality quantifiable data. Imaging western blots and protein gels on the licor. Licor odyssey clx amersham typhoon nir fluorescence. If you suspect a problem with the instrument, the user diagnostic can also be used to verify a problem before contacting technical support.
We have 1 licor odyssey manual available for free pdf download. Western blotting wb is an analytical technique originally developed in the late 1970s to determine the presence or absence of a protein of interest in a complex biological sample, such as a tissue. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and remodelling. Transfer to a membrane licor, protein electrotransfer methods 4. Analyzing western blots with image studio lite february 02, 20 image studio lite is a free software package from li cor biosciences aimed at life scientists that want to analyze gels, western blots, dot blots, and other similar lab outputs.
Instrument utilities and diagnostics user diagnostics if you have a problem with your odyssey imager, licor technical support staff may ask you to perform the user diagnostic function. Blots were scanned by licor odyssey infrared imaging system and analyzed by image j 1. A guide to modern quantitative fluorescent western blotting. All users are responsible for the proper use, according to defined protocol, when using the li. In addition, software functions related to the odyssey mousepod accessory are discussed in the odyssey in vivo imaging guide. Which is the best and more sensitive western blot imaging system. Ge lifesciences molecular dynamics ge healthcare typhoon 9410 variable mode imager unites proven storage phosphor autoradiography technology with fourcolor nonradioactive fluorescent labeling. Operators manual licor odyssey operators manual 29 pages. Licor, odyssey infrared imaging system detecting infrared excited and emitted signals high sn ratio from membranes of western blots, protein arrays, micorplates of incell western assays, oncell.
For measurements of gas exchange between terrain and the atmosphere, licor eddy covariance solutions combine precision instrument platforms, flux computation systems. Tested connection to software through tplink switch. Chapter 2 of the odyssey user guide describes how to start scans and set the scanning parameters. Western blot detection support getting started thermo. Featuring miniaturized electronics and simplified hardware, the newlyredesigned li7500ds open path co 2 h 2 o analyzer requires only 4 watts of power during normal operationlower than any other ec gas analyzer available. Imaging western blots and protein gels on the licor odyssey. The odyssey clx imager is intended to be used by qualified personnel. Page 1 the safety of this product can not be guaranteed if the product is used in any other way than is specified in this manual. Staining of j774 lysate with human anti actin beta hca147 followed by horseradish peroxidase conjugated mouse anti streptag antibody, clone streptag ii staining of hela whole cell lysate with. The safety of this product can not be guaranteed if the product is used in any other way than is. Licor aerius 9250 automated infrared imaging system ebay. Western blotting accessories licor blot washer configured for washing and secondary antibody incubation of four blots in incubation boxes. Western blotting accessories licor blot washer configured for washing and secondary antibody incubation of four. By using digital images, strong and weak bands are detected on the same blot, saving labs time by not waiting on film to develop or the uncertainty whether a weak band requires multiple exposures to confirm.
Chemiluminescence similar sensitivity to film resolution. Ive used it in multiple applications and have noticed a higher quality output compared to traditional techniques. Licor biosciences introduces nearinfrared small animal. The odyssey infrared imaging system allows multiplex western blotting and accurate protein detection and quantification using secondary antibodies labeled. Measure using a licor odyssey scanner in the 700 nm channel or measure od at 595 nm in a standard microplate spectrophotometer. Gpi tm150bp manuals and user guides, measuring instruments. All steps were performed as described in the user manual of the kit.
Can be used to distinguish between normal and cancer cells. Gel and blot imaging shared resources fred hutchinson. Quantitative gel and blot imaging and analysis shared. Two different wavelengths for western blot fluorescent dyes 680 and 800nm and pcr gels dyed with gel. Used other licor odyssey imager for sale dotmed listing. Jun 12, 2008 the pearl imager joins li cor s line up of imaging systems for dna, small animal, and proteomic research including the odyssey imaging system and mousepod, which also offers researchers a costeffective approach to in vivo small animal imaging. Scientific fluorescence scanner for gel and blot imaging. All users are responsible for obtaining the proper approval and training before the use of the li. Be aware of the hazards associated with any chemical before using it. Licor products have been designed to be safe when operated in the manner described in this manual.
Please use the protocol mentioned on page 14 of the manual when using the ibind flex western system in conjunction with the licor odyssey imaging system. Mar 10, 2015 the blots were washed, treated with a secondary antimouse alexa 680 nm conjugate molecular probes and imaged using a li cor odyssey imager. The availability of licor odyssey antibodies makes detection of multiple proteins very easy. The odyssey fc imager is intended to be used by qualified personnel. Odyssey digital imaging accessories pdf manual download. The licor odyssey scanner is recommended for two color fluorescence imaging of western blots and in cell westerns microplates in far red and near infrared channels. Odyssey clx application and technical notes show the versatility of the clx. Licor odyssey 9120 imaging system the lab world group. Odyssey fcimager placementinthelaboratory theodysseyfcimagerweighsapproximately27kg60lbs. Printinghistory publicationnumber97716222 printedjune2016 globalheadquarters servingtheunitedstates4647superiorstreetlincoln,ne68504 tollfree.
The licor odyssey fc imaging system is easy to use and the operation is selfexplanatory. Licor biosciences, clx operators manual, clx instructions manual, clx, clx instrument manual created date. However, the sapphire biomolecular imager is a next generation laser scanning system which offers the best of both worlds. The blots were washed, treated with a secondary antimouse alexa 680 nm conjugate molecular probes and imaged using a licor odyssey imager. Nrb common equipment neuroscience suny upstate medical. Information on odyssey software can be found both in this manual and in the odyssey user guide. Full western blot images are shown in supplementary. All images are acquired using the li cor odyssey classic imager and associated image pro analysis software version 3. Licor odyssey scanner certified with warranty for sale. Licor aerius 9250 automated infrared imaging system. Operators manual odyssey licor operators manual 2 pages. Licor odyssey clx infared imaging system enables twocolor detection and quantification of fluorescent dyelabeled antibodies room 3721 lonza 4dnucleofector, core, xunit, yunit enables highly efficient. Prepare samples and determine protein concentrations.
The page provides a catalogue of brands and devices, each offering to view or. The sapphire biomolecular imager quantitative western blots. A multi user site license for imagequant tl iqtl is available for the analysi sof typhoon data. The odyssey imager should not be used with any radioactive materials.
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